Journal: Science Advances

Article Title: The tumor suppressor adenomatous polyposis coli regulates T lymphocyte migration

doi: 10.1126/sciadv.abl5942

Figure Lengend Snippet: Primary and secondary antibodies/reagents used for immunofluorescence.

Article Snippet: APC anti-human CD11a , Mouse IgG1 , Miltenyi Biotec 130-127-294 , 1:50.

Techniques: Immunofluorescence, Purification

Journal: Science Advances

Article Title: The tumor suppressor adenomatous polyposis coli regulates T lymphocyte migration

doi: 10.1126/sciadv.abl5942

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: APC anti-human CD11a , Mouse IgG1 , Miltenyi Biotec 130-127-294 , 1:50.

Techniques: Cytometry

Journal: Science Advances

Article Title: The tumor suppressor adenomatous polyposis coli regulates T lymphocyte migration

doi: 10.1126/sciadv.abl5942

Figure Lengend Snippet: Antibodies used for Western blot.

Article Snippet: APC anti-human CD11a , Mouse IgG1 , Miltenyi Biotec 130-127-294 , 1:50.

Techniques: Western Blot

Journal: Cell Communication and Signaling : CCS

Article Title: Neutrophil membrane-coated circular RNA nanoparticles for targeted immunotherapy in HER2-positive breast cancer brain metastasis

doi: 10.1186/s12964-025-02321-w

Figure Lengend Snippet: Construction and Characterization of HER2-Targeted dHL-60 Cell Membranes. (A) Schematic representation of the lentiviral vector design used to overexpress the scFv targeting HER2. The vector includes the anti-HER2 scFv, a membrane localization signal, a FLAG tag for detection, a hinge region for flexibility, and a transmembrane domain to anchor the scFv to the cell membrane. (B) SPR analysis illustrating the binding affinity of scFv to the HER2 protein. (C) Flow cytometry analysis comparing the binding of scFv (anti-HER2) to SKBR3 cells versus a control scFv. (D) Flow cytometry assessment of CD11b expression levels in HL-60 cells before and after differentiation induction with DMSO. (E) Western blot results demonstrating the expression of adhesion molecules CD11b, CD18, CD49d, and CD31 in differentiated HL-60 cells. (F) Western blot analysis was performed using an anti-FLAG antibody to detect the FLAG-tagged scFv (anti-HER2). A band corresponding to the scFv-FLAG fusion protein is observed in dHL-60 cells transduced with the lentiviral vector, but not in control cells. (G) Flow cytometry analysis (anti-FLAG antibody) demonstrating the surface expression of scFv (anti-HER2) on dHL-60 cells overexpressing scFv (anti-HER2). (H) Illustration depicting the construction and extraction process of the cell membrane from HL-60 cells, outlining the steps involved in preparing membrane-coated nanoparticles

Article Snippet: CoraLite ® Plus 488, PE-conjugated Goat Anti-Rabbit IgG, antibodies against CD11B, CD49d, CD31, CD18, β-actin, His Tag, FLAG Tag and CXCL9 were obtained from Proteintech (Wuhan, China).

Techniques: Plasmid Preparation, Membrane, FLAG-tag, Binding Assay, Flow Cytometry, Control, Expressing, Western Blot, Transduction, Extraction

Journal: Frontiers in Immunology

Article Title: Genetic Engineering and Enrichment of Human NK Cells for CAR-Enhanced Immunotherapy of Hematological Malignancies

doi: 10.3389/fimmu.2022.847008

Figure Lengend Snippet: NK cells rely on endogenous IL15 production for in vivo persistence in NSG mice. (A) Schematic representation of the vector maps of the IL15 constructs used for NK cell transduction. (B) Primary human NK cell preparations consisting of 50% EGFP/IL15 or EGFP/IL15-IL15R and 50% untransduced NK cells (or 100% untransduced cells as control group) were injected into busulfan-preconditioned mice (3 animals per group). On days 5, 10, 20, and 27, NK cell persistence in the bloodstream was analyzed by mouse CD18, human CD45, and CD56 staining as well as EGFP expression. (C) Example of gating strategy showing representative data from day 10. Values indicate mean ± SEM of all three animals. (D) NK cell persistence in the blood including ratios of transduced to untransduced NK cells. Data are represented as mean ± SEM. Significances of p<0.05 are indicated by an asterisk (*).

Article Snippet: Peripheral blood was analyzed at days 5, 10, 20, and 27 after lysis of erythrocytes with BD Pharm Lyse (BD Biosciences, San Jose, CA, USA) on a MACSQuantTM Analyzer 10 for murine CD18, human CD45, CD56, and EGFP-expressing cells (all antibodies were REA clones from Miltenyi Biotec) as described previously ( ).

Techniques: In Vivo, Plasmid Preparation, Construct, Transduction, Control, Injection, Staining, Expressing